Human deoxyribonuclease I (DNase-I) ELISA Kit DEIA-CL026 from Creative Diagnostics

Product Specs
ItemHuman deoxyribonuclease I (DNase-I) ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIA-CL026
Quantity96T
Detection Range1.56 ng/ml-100 ng/ml
Sample Typeserum, plasma, tissue homogenates
SensitivityThe minimum detectable dose of human DNase-I is typically less than 0.39 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations
ApplicationsELISA
ReactivityHuman
Components1. Assay plate (12 x 8 coated Microwells): 1 (96 wells)
Opened kit: May be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.

2. Standard (Freeze dried): 2
3. Biotin-antibody (100 x concentrate): 1 x 120 µl
4. HRP-avidin (100 x concentrate): 1 x 120 µl
Opened kit: May be stored for up to 1 month at 2 - 8°C. If don't make recent use, better keep it store at -20°C.

5. Biotin-antibody Diluent: 1 x 15 ml
6. HRP-avidin Diluent: 1 x 15 ml
7. Sample Diluent: 1 x 50 ml
8. Wash Buffer (25 x concentrate): 1 x 20 ml
9. TMB Substrate: 1 x 10 ml
10. Stop Solution: 1 x 10 ml
Opened kit: May be stored for up to 1 month at 2 - 8°C.

11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1
PrecisionIntra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess

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Creative Diagnostics

45-1 Ramsey Road

Shirley, N 11967

United States

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Human deoxyribonuclease I (DNase-I) ELISA Kit from Creative Diagnostics

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Creative Diagnostics

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Human deoxyribonuclease I (DNase-I) ELISA Kit from Creative Diagnostics

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